If patient group is РІР‚ВOРІР‚в„ў then label another tube for РІР‚ВOРІР‚в„ў cells.

NOTE : PatientРІР‚в„ўs who need only ABO/Rh performed shall have РІР‚ВOРІР‚в„ў cells in their back type as screen for Cold type antibodies, auto-antibodies and Bombay phenotype.

Grade and record results in the medical software.

Examine for agglutination macroscopically under agglutination viewer.

Mix the contents of each tube thoroughly and centrifuge immediatelyР’В at calibrated speed and time.

Place 1 drop of reagent A 1Р’В and B reagent cells into tubes РІР‚Вa 1РІР‚в„ў and РІР‚ВbРІР‚в„ў.

Now add 1 drop of patientРІР‚в„ўs red cell 5% suspension to tube РІР‚ВAРІР‚в„ў and РІР‚ВBРІР‚в„ў.

Add 1 drop (50ul) of each blood-grouping reagent to tube РІР‚ВAРІР‚в„ў and РІР‚ВBРІР‚в„ў and add 2 drops of patientРІР‚в„ўs serum in tubes РІР‚Вa 1РІР‚в„ў and РІР‚ВbРІР‚в„ў.

Label 4 test tube with patientРІР‚в„ўs complete MR#/OPD# or donor#.

Centrifuge the clotted specimen that clears serum is obtained.

Prepare 5% suspension of patientРІР‚в„ўs red cells.

Refer to quality control procedures for detail. To confirm the reactivity of anti-sera, it is recommended that these reagents be tested each day of use with antigen positive and negative red cells. Samples can be tested up to 3 days from the date of collection. Red cells from the EDTA anti-coagulated are best suited for front type and clotted sample is suitable for back typing.

All reagents shall be considered as potentially hazardous.

Turbidity may indicate reagent deterioration or contamination.

NOTE : PatientРІР‚в„ўs who need only ABO/Rh performed shall have РІР‚ВOРІР‚в„ў cells in their back type as screen for Bombay phenotype. Agglutination is suggestive of presence of corresponding antigen and no agglutination indicates absence of antibody.

The ABO group of red cells is determined from the pattern of reactivity obtained with test reagents.Р”В«ack/Reverse type:Р’В Antibodies present in patientРІР‚в„ўs serum reacts with the antigen on red cell. Front/Forward type:Р’В Direct agglutination of red cells with a particular reagent indicates the presence of the corresponding antigen.

A negative blood type panel